EVALUATION OF ANTIOXIDANTS ACTIVITY CASSIA BIFLORA

Rahul Kumar, *Dr. Esha Vatsa, Krati, Nidhi Chaudhary and Dr. Amandeep Singh

This study investigates the antioxidant potential of Cassia biflora, a medicinal plant traditionally used for various therapeutic purposes. Preliminary phytochemical screening revealed the presence of bioactive compounds known for their antioxidant activity, including flavonoids, phenolics, tannins, alkaloids, and saponins. The antioxidant capacity of the plant extract was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay, measured spectrophotometrically at 517 nm. The extract exhibited a concentration-dependent increase in radical scavenging activity, comparable to that of ascorbic acid, a standard antioxidant. A notable IC₅₀ value indicated significant antioxidant efficacy. These findings suggest that the antioxidant potential of Cassia biflora is likely attributable to its rich phytochemical composition. Further studies are recommended to isolate and characterize the active constituents for potential therapeutic applications.