DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROSCOPIC METHOD FOR THE ESTIMATION OF MESALAMINE

Sruthi K., Anusree M., Arathi Vinod A.*, Sneha K., Sneha V. P.

A simple, accurate, precise, and cost-effective colorimetric method was developed and validated for the quantitative estimation of mesalamine in bulk drug and pharmaceutical dosage forms. The proposed method is based on the formation of a colored complex, which shows maximum absorbance at 406 nm when measured using a UV–Visible spectrophotometer. The method obeys Beer–Lambert’s law over the selected concentration range, exhibiting good linearity with a correlation coefficient of 0.9953. The developed method was validated as per ICH guidelines for parameters such as linearity, precision, accuracy, and reproducibility. The results obtained demonstrated that the method is reliable and suitable for routine analysis of mesalamine. Due to its simplicity, sensitivity, and economic advantage, the proposed colorimetric method can be effectively applied for quality control analysis in pharmaceutical laboratories.