COMPARATIVE QUANTITATIVE ESTIMATION OF β SITOSTEROL IN SELECTED INDIAN MEDICINAL PLANTS BY HPTLC FINGERPRINTING

Chaurasia Sarita*, Yadav Saroj, Swathi B.

Background: Herbal plants are abundant in phytochemicals and these constituents plays promising role in the treatment and management of different diseased conditions. Different scientific methods were used for qualitative and quantitative estimation of these phytoconstituents to use these medicinal plants as therapeutic agents. In this study, investigation of β sitosterol in all selected plants was done by HPTLC fingerprinting. Objectives: Present research was aimed to do comparative quantitative estimation of β sitosterol in all selected Indian medicinal plants by using reference biomarker with the help of HPTLC fingerprinting. Materials and methods: Based on significant use in traditional medicine, the four Indian medicinal plants were taken. All the dried plant part materials were subjected for extraction by using the Soxhlet with a mixture of ethanol and distilled water (70:30) at temperature 60-70 ^oC for 24 hours. The extracts were concentrated using Rota evaporator. Redissolved methanolic extracts of all selected plants and reference biomarker β sitosterol were used for HPTLC analysis in mobile phase Toluene: Ethyl acetate (8:2) at λ=540nm by using CAMAG Switzerland and densiometric estimation was done with CAMAG TLC scanner and Win cat software. Results: HPTLC chromatogram for β-sitosterol was analyzed and Rf value for standard β-Sitosterol was found 0.53, and the area of the peak of β- sitosterol was 7783.39 at a concentration (20μg/10μl). Maximum concentration and peak area are obtained for extract of stem of Tinospora cordifolia Miers with Rf value 0.52 and area of peak 2229.57 (% β- sitosterol-1.30). Extract of roots of Boerhavia diffusa L. with Rf value 0.53 and area of peak 1879.40 (% β- sitosterol: 0.92), extract of leaves of Moringa oleifera Lamk with Rf value 0.53 and area of peak 653.2 (% β- sitosterol: 0.38) whereas β- sitosterol is not detected in hydroalcoholic extract of Ficus religiosa L. Conclusion: Results of present research on HPTLC analysis of all selected plant parts provide a tool for authentication, standardization and quality control parameters of all selected species. Values obtained by present research may be helpful for other researchers in future.