HISTOLOGICAL STUDY OF THE EFFECT OF ACETAMINOPHEN DRUG ON THE KIDNEYS OF MALE ALBINO RATS

Zainab Salah Abdulgabar, Maysoon Khudair AL- Hadrawi*, Rajaa Jawad Mohamed

The study was performed in the animal house of the College of Education, for a period (21/11/2021- 12/12/2021) to evaluate the histological changes in the kidney of a male albino rat, treated with acetaminophen (450mg/kg). the study lasted two months by Following (20) male rats and was (16-20) weeks weighted between (200-250g) they divided into two groups each consisting of ten males. The first group was orally given the tab water and it was the control group. The second group treated and It was orally with acetaminophen at (450 mg/kg) Five rats from each group were then left to be fed with water and diet for two weeks after the last treatment in order to monitor the progress of clinical signs, histological and macroscopic changes. Hematoxylin and Eosin stained images showed that acetaminophen overdose induced acute kidney injury as demonstrated by widening of glomeruli space (Bowman space), tubular dilatation, numerous cellular debris in the renal tubules with tubular epithelial degeneration, and vacuolization the pathological changes were observed in the histological section of kidneys that dosed ((acetaminophen) such as cellular change in the glomeruli cells, the epithelium of was disrupted and increased connective tissue, From this study, It was concluded that acetaminophen drug has negative effects in the cells of the kidneys tissue, For long term. Despite the fact that widespread use of acetaminophen it made histopathological changes in the kidneys. These findings revealed nephrotoxicity was associated with an increase in oxidative damage and apoptosis.