THE IMMUNOMODULATOR ACTIVITY OF NANOSILVER’S INULIN BASED ON IMMUNOGLOBULIN G LEVEL OF MICE INDUCED BY MEASLES VACCINE

Dian Eka Ermawati*, Putri Indah Nuraini, Niki Putri Anjani, Adi Yugatama

Vaccination is an effort to increase a person's immunity against a disease actively. The process of the formation of antibodies by the body after vaccination requires complementary treatment for immunostimulators. Silver in the medical field developed as a therapeutic agent. When in the form of nanoparticles, it can reduce toxicity and act as an immune catalyst. Inulin acts as a bioreductant to synthesize silver because it is more environmentally friendly than physical and chemical methods. Nanosilver biosynthetic (AgNPs) using gembili inulin confirm as an antibacterial agent. This study aims to determine the immunomodulator activity of nanosilver based on Immunoglobulin G of Balb/c mice induced by the measles vaccine and the stability in 21 days. The research method was experimental in the laboratory. AgNPs biosynthesis process using inulin gembili as a bioreductant, then characterize, animal conditioning, treatment of an animals, ELISA test, and stability test of nanosilver. The data was analyzed statistically using one-way ANOVA. The results of the ELISA readings showed an increase in the Optical Density (OD) value of the nanosilver sample at a dose of 4 mg/kg BW was higher than the other treatments but not significantly different with the herbal stimulant. There is no effect of storage time on the stability of nanosilver during 21 days at a temperature of 4 °C on the maximum wavelenght shift. However, it is still in the nanoparticle size range. In addition, there is a change in the color of the solution from light brown to dark brown, which indicates that a nanosilver formation process has continued. It is necessary to confirm the acute toxicity test of the nanosilver in the future.