CYPERMETHRIN CAUSE ALLERGIC INFLAMMATORY RESPONSE IN RAT LUNG

González-Ramírez Cristina, Ramírez-López Elsa Marcela, Aldana-Madrid María Lourdes, Ventura-Juárez Javier, Muñoz-Ortega Martin Humberto, Vásquez Mellado-Cerrillo Carolina, Martínez-Saldaña Ma. Consolación*

Cypermethrin is a type 2 photosensitive liposoluble pyrethroid for agricultural and domestic use, has a half-life in the ambient air for days, its metabolism in human liver generate a cyan group (CN), an alcohol fraction (dimethylcyclopropanecarboxylic acid) (DCCA), and 3-acid phenoxybenzoic acid (3-PBA) with reactive oxygen species (ROS) properties, there are scarce studies when is inhaled. The objective of this work was to evaluate the structural changes and activation of allergic inflammatory response in lower airway components and alveoli induced by inhaled exposure to low doses of cypermethrin. Rats were exposed during 7, 14 and 21 days by inhalation, then were analyzed the serum levels of IgE by ELISA, performed H&E stain on lung tissue for analyze the structural changes, through immunofluorescence were detected positive cells to IL-4, IL-5 and IL-13, finally by qPCR was quantified mRNA for IL-4, our results shows that cypermethrin induce a local and systemic allergic inflammatory reaction in rats, specifically in lungs, induce the production and secretion of products of Th2 cells (IL-4, IL-5, IL-13), eosinophils and, high serum levels of IgE, demonstrating like this, the cytotoxic effect of cypermethrin at 7 and 14 days exposition.