COMPARATIVE ANALYSIS OF THE LEVELS OF PROTEIN S100 AND NEURON-SPECIFIC ENOLASE (NSE) IN PEDIATRIC PATIENTS BEFORE ANESTHESIA WITH SEVOFLURANE AT ANNUAL AGE AND REFERENCE VALUES

V. Demjanski*, M. Sholjakova, S. Litajkovska, LJ. Donev and LJ. Mikjunovikj

Background: Neuron-specific enolase and protein S100 are serum biomarkers that indicate the presence of neurogenic damage. The awareness that sevoflurane affects serum levels of neuron-specific enolase (NSE) and protein S100 has increased interest in these markers. Methods: Conducted over a year with 70 pediatric patients under sevoflurane anesthesia, the study assessed preoperative S100 and NSE levels, comparing them to references and analyzing deviations by age and gender. Electrochemiluminescence immunoassay (ECLIA) performed laboratory analyses, and SPSS software facilitated statistical evaluation. The study aimed to provide insights into pediatric preoperative S100 and NSE levels, comparing them to reference ranges and exploring factors influencing deviations. Results: In a study encompassing 70 pediatric patients (3 months to 14 years) undergoing sevoflurane anesthesia, neuron-specific enolase (NSE) averaged 18.40±6.22 ng/ml. Gender analysis disclosed higher NSE in males (19.08±5.95) compared to females (15.69±6.78). Protein S100 mean was 0.09±0.08 ug/L, with notably higher values in males (0.09±0.09) than females (0.07±0.04). Conclusion: Preoperative NSE and S100 levels in pediatric patients under sevoflurane anesthesia exhibit variations influenced by age and gender. Establishing gender-specific reference values is suggested for accurate assessment. The study's limitations include a small sample size, emphasizing the need for future research with larger cohorts for comprehensive insights.