HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY OF SIMULTANEOUS ESTIMATON OF CILNIDIPINEHYDROCHLORIDE AND CHLORTHALIDONE IN ITS COMBINED DOSAGE FORMS

*Raveendran K. C. and Seethalp S.

Hypertension is a major health problem throughout the world because of its high prevalence and its association with increased risk of cardiovascular disease. High blood pressure (BP) or HTN has been recognized by the world health organization (WHO) as the most important preventable cause of premature morbidity and mortality in developed countries. HTN is a condition which probably requires lifelong treatment and life style modifications. The HPTLC instrument used in this purpose having a twin trough chamber, Camage UV-TLC plate scanner, video densitometer. The mobile phase was a combination of Ethyl acetate :Hexane in the ratio of 6:4 v/v stationary phase was HPTLC recoated plate of Silica gel G60F254.Scanning wavelength was 235 nm. The Rf values were obtained as 0.98 for Cilnidipine and 0.77 for Chlorthalidone. Assay result was found to be 101.40%w/w for Cilnidipine and 99.94%w/w for Chlorthalidone (Area wise). Limit of detection and Limit of Quantification values were obtained as 0.3956 ng and 1.1980ng (Area wise) respectively.