ABSTRACT
GCMS ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS AND SCREENING FOR ANTI-ANAEMIC ACTIVITY OF THE METHANOLIC EXTRACT OF AMARANTHUS CAUDATUS LEAVES
Farha Fatma* and Dr. Arun Kumar
The present study was aimed to determine phytochemical constituents with the aid of Gas Chromatography Mass Spectroscopy technique in methanolic extract of locally grown A. caudatus leaves for their anti-anaemic activity in Ranchi district of Jharkhand. The bioactive compounds present in methanolic leaves extract, identified by GCMS chromatogram showed 55 peaks indicating the presence of 55 bioactive compounds. The plant contains large amount of secondary metabolites having wide range of biological activities on physiological system. 2-Ethylbutyric acid, eicosyl ester (9.20%) was found to be major compound and fifty four other major compounds such as 4 H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (8.51 %), n-Hexadecanoic acid (8.47%), Phytol (7.28%), 1,2,3,-Propanetriol (6.70%), Chondrillasterol (6.51%) and remaining compounds with peak area ranging from 5.26 % to 0.21% were found to be present. Herbal medicines were formulated and given to some patients. Pre and post haemoglobin level was measured using a haemocytometer. There was an increase in the level of haemoglobin after consumption of herbal powder. The result proved ability of this plant in curing nutrition deficiency anaemia. Medicinal plants are backbone of traditional healthcare system and anti-anaemic activity of the plant is due to presence of different bioactive compounds. They can also act as anti-oxidants and beneficial in management of hypertension, heart diseases, stroke and heart failure etc.
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