GCMS ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS AND SCREENING FOR ANTI-ANAEMIC ACTIVITY OF THE METHANOLIC EXTRACT OF MORINGA OLEIFERA LEAVES

Farha Fatma* and Dr. Arun Kumar

The present study was aimed to determine phytochemical constituents with the aid of Gas Chromatography Mass Spectroscopy technique in methanolic extract of locally grown M. oleifera leaves for their anti-anaemic activity in Ranchi district of Jharkhand. The bioactive compounds present in methanolic leaves extract, identified by GCMS chromatogram showed 42 peaks indicating the presence of 42 bioactive compounds. It revealed 1,2,3-Propanetriol be the major phytocompound present in Moringa followed by 2-Ethylbutyric acid, nonyl ester (9.44%), Phytol (6.89%), Hexadecanoic acid, 2-hydroxy-1-(hydroxymethy) ethyl ester (6.32%), 2-Ethylbutyric acid, eicosyl ester (5.16%) and other remaining compounds ranging from 4.76% to 0.21%. Herbal powder was formulated and given to some patients. Pre and post haemoglobin level was measured using a haemocytometer. There was an increase in the level of haemoglobin after consumption of herbal powder. The result proved ability of this plant in curing nutrition deficiency anaemia. Medicinal plants are backbone of traditional healthcare system and anti-anaemic activity of the plant is due to presence of different bioactive compounds. They can also act as anti-oxidants and beneficial in management of hypertension, heart diseases, stroke and heart failure etc.