IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF LEAF EXTRACT OF ARISTOLOCHIA BRACTEOLATA

S. Selvakumar*, N. Chidambaranathan and R. Sarojini

Aristolochia bracteolata is an important medicinal herb and it is an original of Indian subcontinent and has become naturalized in the tropical and sub-tropical areas around the world. The plant is usually gathered from the wild and is used locally in traditional medicine. It is sometimes cultivated for medicinal use in India. In the indigenous system of medicine, the plant was used as purgative, antipyretic & anti-inflammatory agents. The root part has antifungal and antibacterial activity and was used to treat syphilis, gonorrhoea, and skin diseases and also used during labours to increase uterine contraction. Its leaves are bitter and anti-helmintic, antiulcer, anti-plasmodial and are medicinally important. The whole plant is very bitter and has abortifacient, alterative, anthelmintic, antiperiodic, emmenagogue and purgative properties. In the current investigation, Leaf extract of Aristolochia bracteolata has shown anti-inflammatory and antioxidant properties. Aristolochia bracteolata ABETpowder extract has shown significant in vitro antioxidant activity by DPPH assay in the current investigation. At 100 mg/ml ABET, the average inhibition percentage is 67%, with the IC50 value was found to be 216.7884 mg/ml compared to the standard ascorbic acid IC50 value of 33.7334 mg/ml. Further investigation of in vitro anti-inflammatory activity by the protein denaturation method compared to the standard. At 100 mg/ml, ABET has an average inhibition percentage of 40%, with the IC50 value was found to be 296.1292 mg/ ml compared to the standard IC50 value of 121.29 mg/ml.Overall investigation results, such as ABET, had good antioxidant and moderate anti-inflammatory activity.