DETERMINATION OF λ-MAX AND LINEARITY FOR OMEPRAZOLE AND ASSAY METHOD FOR RABEPRAZOLE TABLETS I.P. USING UV-VIS SPECTROSCOPY

M. Mohanapriya*, A. Arun, G. Girish Kumar, V. Nandha Kumar, S. Savin and Dr. R. Manivannan

This study presents the development and validation of UV spectrophotometric methods for the analysis of Omeprazole and Rabeprazole in pharmaceutical formulations. The maximum absorption wavelength (λ-max) of Omeprazole was determined to be 269.4 nm, and the method exhibited excellent linearity across a concentration range of 5–25 μg/mL, with a correlation coefficient (r) of 0.9998. Similarly, the method for Rabeprazole tablet assay was precise and accurate, with a calculated percentage purity of 93.5% w/v, meeting pharmaceutical standards. Both methods demonstrated simplicity, stability, cost-effectiveness, and rapid analysis time, making them ideal for routine quality control. Adhering to ICH guidelines, these methods were validated for reliability, reproducibility, and compliance. The study highlights the suitability of UV spectrophotometry for determining drug concentration in various formulations due to its minimal sample requirements, ease of operation, and cost efficiency. The findings confirm the methods' applicability for routine pharmaceutical analysis and quality control of Omeprazole and Rabeprazole.