THE EFFECT OF 10% ETHYL ACETATE SUBFRACTION OINTMENT OF MENIRAN LEAVES (PHYLLANTUS NIRURI L.) ON THE EXPRESSION OF TNF-Α IN WHITE MALE RATS

Sanubari Rela Tobat*, Satya WydyaYenny, Fatma Sri Wahyuni, Etriyel MYH, Suvina Rahmadani, Mimi Aria, Puspa Pameswari, Epi Supriwardi, Sandra Tri Juli Fendri

Research has been carried out on the effect of giving meniran leaves (Phyllanthus niruri L.) in the form of an ointment with a concentration of 10% on the expression of TNF-α in male white rats. This study aims to determine the effect of giving meniran leaves (Phyllanthus niruri L.) in healing excision wounds assessed by the percentage of wound healing area, epithelialization time and TNF-α expression, on days 5, 10 and 15 of skin tissue. Experimental animals were grouped into 2 groups, namely, group 1 as a control by administering an ointment base (vaseline flavum) and group 2 as a treatment group, namely, by administering 10% meniran leaf (Phyllanthus niruri L.) ethyl acetate subfraction ointment. Both groups were observed and measured the percentage of wound healing, epithelialization time and the expression of TNF-α in inflammatory cell tissues (neutrophils, macrophages, and lymphocytes) on days 5, 10 and 15. The results of data analysis using one-way ANOVAfollowed by the Duncan test showed that there was significant difference between the control group and the treatment group in the percentage of wound healing area, epithelialization time and TNF-α expression (p<0.05), so it can be concluded that the preparation of meniran leaf ethyl acetate subfraction ointment with a concentration of 10% has an effect as excisional wound therapy.