SPECTROPHOTOMETRIC ANALYSIS OF QUERCETIN IN FORMULATIONS

Paul Jacob, Amna Sherin O., Anjana K.*, Karthika K. and Mridhul M.

In present study, the spectrophotometric analysis of quercetin in two marketed formulations using single point standardization and the calibration curve method. Ethanol was used as the solvent, and the absorbance was measured at 372 nm. The analysis was conducted over a concentration range of 2 to 10 μg/ml to determine the quercetin content in the formulations. Different analytical performance parameters such as linearity, precision, accuracy, specificity, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization ICH Q2B guidelines. The calibration curve method allowed for a more accurate quantification of quercetin in the formulations with good r2 (>0.999). The results showed that Formulation 2 exhibited absorbance values that were closer to those of the standard quercetin solution, indicating a more consistent quercetin concentration. These findings underscore the utility of UV spectrophotometry for the quantification and quality control of quercetin in pharmaceutical formulations. This analysis highlights the importance of using reliable methods like UV spectroscopy for ensuring the quality and therapeutic efficacy of quercetin-containing products.