RESEARCH ON EVALUATION OF IN-VITRO ANALGESIC AND ANTI-INFLAMMATORY POTENTIAL OF AGERATINA ADENOPHORA LEAVES EXTRACT

Seema Rawat, Abhishek Bhardwaj*, Krati, Dr. Esha Vatsa, Dr. Amandeep Singh

Ageratina adenophora, a plant known for its traditional medicinal applications, was investigated for its potential analgesic and anti-inflammatory properties. In this study, methanolic extracts of A. adenophora leaves were prepared and subjected to in-vitro assays to evaluate their analgesic and anti-inflammatory activities. The leaves of A. adenophora were dried, powdered, and extracted using the cold maceration technique with methanol. Phytochemical screening of the methanol and chloroform extracts revealed the presence of various bioactive compounds, including alkaloids, flavonoids, phenols, steroids, saponins, tannins, cardiac glycosides, carbohydrates, terpenoids, and proteins. The study aimed to evaluate the in vitro analgesic and anti-inflammatory properties of A. adenophora leaf extract. Various solvent extracts were prepared from the leaves and tested for their analgesic and anti-inflammatory activities using established in vitro methods. The anti-inflammatory activity was determined by assessing the extract's ability to inhibit the activity of cyclooxygenase (COX) enzymes. The results showed that the leaf extracts exhibited significant inhibitory effects on both analgesic and anti-inflammatory mediators, suggesting their potential as an effective, plant-based alternative for managing pain and inflammation.[1,2] Ageratina adenophora, locally known as “kala bansa” in Uttarakhand, India, has been traditionally used for its wound-healing properties. This study aimed to scientifically evaluate its effectiveness in supporting its traditional use as a natural wound healer. The wound-healing potential of A. adenophora ethanolic extract was assessed using excision and incision wound models. A formulation containing the extract was applied to wounds for 13 days. The healing process was monitored by measuring wound area reduction, epithelialization time, and tensile strength. Additionally, a wound index was recorded for both excision and incision models.[3] A. adenophora is a highly invasive plant species known to disrupt ecosystem stability. Studies indicate that this weed releases allelopathic compounds that negatively affect the growth and productivity of food crops. Additionally, it contains toxic substances that can be harmful to animals that ingest it.[4,5]