HERBAL STANDARDIZATION OF FORMULATION CONTAINING CURCUMINOIDS, PIPERINE AND BOSWELLIC ACID BY HPLC

Prajapati Dhrumi*, Patel Pinak and Detholia Krunal

The main emphasis of present work is to develop and validate RP-HPLC method for quantification Curcumin C, Piperine and Boswellic acid along with detection and separation of Curcumin B and C from their combined marketed capsule dosage form.” The separation of components was carried out by using Hypersil ODS C18 column (250*4.6 mm, 5 µm) and the solvent system contains 65 parts of Acetonitrile, 5 parts of Methanol and 30 parts of Water. The flow rate was maintained at 1ml/min and the chromatograms were observed at 245 nm. Eluted components i.e. Curcumin A, B and C, piperine and Boswellic acid has be detected in chromatogram at 2.52, 3.56, 4.16, 5.21 and 8.59 respectively. The linearity was carried out within the concentration range of 30-190, 1-5 and 15-75 µg/mL and the value of r2 were found to be 0.995, 0.995 and 0.998 for Curcumin C, Piperine and Boswellic Acid respectively. Recovery of all three components were found between 96.82 ± 95.33 %. The LOD of Curcumin C was found to be 3.17, for Piperine 0.05 and for Boswellic Acid 1.26 µg/mL. The LOQ for Curcumin C, Piperine and Boswellic Acid was found to be 9.60, 0.17 and 3.81 µg/mL respectively. The developed and optimized method was used successfully for assay of market formulations that incorporated with Curcumin, Piperine, and Boswellia serrata extract.