DEVELOPMENT AND VALIDATION OF A UV SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF IBUPROFEN AND PARACETAMOL IN TABLET FORMULATION

Bhanupratap Ray*, Pradeep Kachhawa and Prof. Ajay Bagherwal

The present review focuses on the development and preliminary validation of a UV-Visible spectrophotometric method for the simultaneous estimation of Paracetamol and Ibuprofen in combined pharmaceutical dosage forms. With the growing prevalence of fixed-dose combinations in therapeutic applications, there is an increasing demand for precise, economical, and rapid analytical techniques. The method employs the simultaneous equation approach based on absorbance measurements at 257 nm for Paracetamol and 221 nm for Ibuprofen, utilizing 0.1N NaOH as the solvent. Calibration curves were constructed over the concentration range of 5–25 µg/mL, demonstrating linearity in accordance with Beer-Lambert’s law. Method validation was conducted in alignment with ICH guidelines, evaluating parameters such as linearity, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), robustness, and ruggedness. The results affirm the method's reliability and suitability for routine quality control of pharmaceutical formulations containing these two active pharmaceutical ingredients.