DPPH RADICAL SCAVENGING ACTIVITY OF THE PURIFIED EXTRACT AND LIQUID-LIQUID EXTRACTION (LLE) FRACTION OF KAEMPFERIA ROTUNDA L.

Dyah Aryantini*

Kunci pepet (Kaempferia rotunda L.) is a plant with potential as a traditional medicine, containing various phytochemical compounds that contribute to pharmacological activities. This study aimed to determine the antioxidant activity of the purified extract and the Liquid Liquid Extraction (LLE) fractions of Kaempferia rotunda L. against DPPH. The rhizomes were extracted via maceration and re-maceration using 70% ethanol. The concentrated K. rotunda extract (KRE) was then purified with n-hexane. The purified extract was further fractionated using LLE with solvents of varying polarity (n-hexane and ethyl acetate). Phytochemical screening was perform qualitatively. The DPPH radical scavenging activity of the purified extract and LLE fractions was assessed using UV-vis spectrophotometry. The yield values obtained from the extraction, purification, and fractionation procedures were as follows: Crude K. rotunda Extract (8.58%), Purified Extract (24.8%), n-Hexane-soluble Fraction (3.81%), Ethyl Acetate-soluble Fraction (30.55%), and Water-soluble Fraction (55.60%) w/w. Phytochemical screening revealed that all samples tested positive for flavonoids. The DPPH scavenging assay showed that the purified extract sample exhibited the strongest activity with an IC50 value of 54.50 µg/mL. The purified extract contained a more varied profile of secondary metabolites compared to the other samples.