THE EFFECT OF 15% ETHANOL EXTRACT OINTMENT OF MENIRAN LEAVES (PHYLLANTUS NIRURI L.) ON HYDROXYPROLINE LEVELS HEALING OF BURN WOUNDS IN WHITE MALE RATS

Sanubari Rela Tobat*, Diana Agustin, Aulia Sari

Meniran (Phyllanthus niruri L.) is a plant that contains phytochemical constituents such as alkaloids, phenolics, flavonoids, terpenoids, steroids, and saponins which can heal burn wounds. This study aims to determine the effectiveness of 15% Phyllanthus niruri L leaf ethanol extract ointment on wound healing. This study was conducted using an experimental method using white male rats with 3 parameters, namely the percentage of wound healing area, epithelialization time, and hydroxyproline levels. Group I was applied with ointment base, group II was applied with T®, and group III was applied with 15% Phyllanthus niruri L leaf ethanol extract ointment. The results showed that the results of the percentage of wound healing area on day 3 of groups I, II, and III were on average 1.17%, 6%, and 3.61%. The results of the percentage of wound healing area on day 7 of groups I, II, and III were on average 13.83%, 34.19%, and 29.54%. The results of the percentage of wound healing area on day 14 of groups I, II, and III were on average 39.41%, 83.93%, and 71.36%. The average epithelialization time of groups I, II, and III was on day 10, day 7, and day 7. The results of the percentage of hydroxyproline levels on day 3 of groups I, II, and III were on average 10.48%, 19.92%, and 17.59%. The results of the percentage of hydroxyproline levels on the 7th day of groups I, II, and III were on average 11.42%, 28.02%, and 20.77%. The results of the percentage of hydroxyproline levels on the 14th day of groups I, II, and III were on average 12.88%, 42.62%, and 20.95%. The results of statistical analysis using one way and two way ANOVA followed by Duncan test (SPSS 25.0) showed a significant difference in groups I, II and III on the percentage of wound healing area and hydroxyproline levels. Epithelialization time (p>0.05), it can be concluded that groups I, II and III were not significantly different. The 15% of ethanol extract of Meniran leaf ointment is quite effective in the percentage of wound healing area, epithelialization time, and hydroxyproline levels in male white rats.