EVALUATION OF INVITRO ANTIOXIDANT ACTIVITIES OF ACETONE EXTRACT OF MUSSAENDA PHILIPPICA FLOWER

Velayutham Mani Mala*, P. Esakki Raja, R. K. Arul Kishore, A. Aravindan, M. Mariselvi, M. Ramasamy, Arun Murugesan, B. Sathiyarajan

Aim: To evaluate the In-vitro antioxidant activity of Mussaenda philippica flowers in acetone extract. Methodology: The shade dried flower plant powder was extracted with acetone by continuous hot percolation method using Soxhlet apparatus. The antioxidant activity was determined by DPPH assay and FRAP assay. Results: At the DPPH assay concentration of 50 mg/ml, the DPPH assay revealed an inhibition percentage of 67%, with an IC50 value of 8.72 mg/ml, in contrast to the standard ascorbic acid, which had an IC50 value of 33.7334 mg/ml. This indicates that MP ET antioxidant activity via the DPPH assay is GOOD when compared to Vitamin C. Further Investigation, MP ET included dose-dependent antioxidant properties within in-vitro FRAP assays. When placed against the standard ascorbic acid in comparable settings. The extract displayed SIGNIFICANT activity at a concentration of 100 mg/ml (1.62- OD) when in comparison to normal Vitamin C. Conclusion: An acetone extract of Mussaenda philippica flowers having more effective antioxidant activity. These in- vitro assays indicate that this plant extract are better source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.