IN VITRO ASSESSMENT OF THE ANTIDIABETIC ACTIVITY OF TRIGONELLA FOENUM-GRAECUM SEED EXTRACT BY ALPHA-AMYLASE INHIBITION METHOD

Dr. Malepati Sandhya Rani*, G. Mounica, H. Bharathi, H. Rakshitha, K. Shannu, K. Devi

Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia resulting from impaired insulin secretion, insulin resistance, or both. The growing global burden of diabetes necessitates the exploration of safer and cost-effective therapeutic alternatives. The present study aimed to evaluate the in vitro antidiabetic activity of Trigonella foenum-graecum seed extract using the alpha-amylase inhibition assay. Methanolic extraction was performed by maceration, followed by qualitative phytochemical screening. The inhibitory potential was assessed at various concentrations and compared with the standard drug acarbose. The extract exhibited significant concentration-dependent inhibition, with 67.88 percent inhibition observed at 60 microgram per milliliter, comparable to acarbose (69.49 percent). The results indicate that fenugreek seeds possess promising antidiabetic potential, supporting their traditional use. Further in vivo and clinical investigations are recommended to validate therapeutic applicability.