EVALUATION OF LIQUORICE (GLYCYRRHIZA GLABRA) EXTRACT AS AN ANTI-INFLAMMATORY AGENT USING IN-VITRO PROTEIN DENATURATION ASSAY

Dr. N. Charitha*, A. Roopa, T. Likitha, V. Harshavardhini, V. pavithra, N. Hemalatha

Inflammation is a protective biological response to harmful stimuli; however, persistent inflammation contributes to chronic disorders. The present study aimed to evaluate the in-vitro anti-inflammatory activity of Glycyrrhiza glabra root extract using a heat-induced protein denaturation assay. The extract was prepared by maceration and tested at concentrations of 125, 250, 500 and 1000 microgram per milliliter. Bovine serum albumin was used as the protein substrate, and diclofenac sodium served as the standard drug. The absorbance was measured spectrophotometrically, and percentage inhibition of protein denaturation was calculated. The extract exhibited concentration-dependent inhibition with maximum inhibition of 68.75 percent at 1000 microgram per milliliter. The results indicate that Glycyrrhiza glabra possesses significant in-vitro anti- inflammatory activity. The findings support its traditional use in inflammatory conditions and suggest its potential as a natural alternative for managing inflammation.