IN-VITRO INVESTIGATION OF Psidium guajava LEAF EXTRACT AGAINST EPILEPSY

Dr. Rajasekaran S., Dr. S. V. Rekha, Amana Jasly, Amina Suhila N., Fathima Shamila*, Mirfa Bathool P. P.

This study investigates the in-vitro antiepileptic potential of Psidium guajava leaf extract. Epilepsy is a chronic neurological disorder characterized by recurrent seizures, and current antiepileptic drugs often cause adverse effects and incomplete seizure control. Guava leaves contain bioactive compounds such as flavonoids, tannins, and phenolics with antioxidant and neuroprotective properties. In this study, dried and powdered leaves were extracted using a suitable solvent and subjected to preliminary phytochemical screening. The antiepileptic activity was evaluated using neuronal cell line models, PC12 (rat pheochromocytoma) and N2a (mouse neuroblastoma), with glutamate-induced excitotoxicity. Cytotoxicity and neuroprotective effects were assessed using the MTT cell viability assay. Results showed that Psidium guajava leaf extract exhibited protective effects against glutamate-induced neuronal damage, indicating potential antiepileptic and neuroprotective activity in vitro.