AN INVITRO ANTI-OXIDANT ACTIVITY OF GENTISIN – AN ACTIVE FRACTION FROM GENTIANA LUTEA

Farija K. H., Hiba Nowrin C. M., Rumana Fathima A., Shifana P. S.*, Shifna Sherin C. P., Sree Lakshmi R., Dr. S. V. Rekha

This study evaluates the in vitro antioxidant activity of gentisin, a bioactive xanthone derivative isolated from Gentiana lutea. The antioxidant potential of gentisin was assessed through several assays: DPPH radical scavenging, nitric oxide and hydroxyl radical scavenging, hydrogen peroxide scavenging, and total antioxidant capacity. Additionally, the MTT assay was conducted to assess cell viability and cytotoxicity in HepG2 cells. The results indicate that gentisin effectively scavenges free radicals and protects cells from oxidative stress-induced damage, supporting its potential as a natural antioxidant and hepatoprotective agent.