METHOD DEVELOPMENT AND VALIDATION OF OMEPRAZOLE IN BULK AND MARKETED FORMULATION BY USING UV-SPECTROPHOTOMETRY

Chinababu D., Vishal Bharat Babar, Mane Yogita S.*, Shevale Harshala S., More Nikita Y., Kokane Sonali P., Rakh Shital S., Aleesha SK

Omeprazole was estimated using a straightforward, sensitive, and selective UV approach that was devised and verified. The technique was developed using UV spectrometry; the optimal concentration was 8µg/ml, the absorbance was 0.428, and the λmax was reached at 301.00 nm. The linearity range of the test technique, which had an R2 value of 0.998, was 0.5 µg/ml to 16 µg/ml. It was discovered that the precision and accuracy percentage RSD ranged from 0.23 to 1.67. The results indicated that the intraday precision was 0.26 and the interday precision (%RSD) was 0.22. The accuracy (recovery percentage) was found to be within the acceptable range of 99.89–100.21%. The developed method's LOD and LOQ were 0.061µg/ml and 0.186 µg/ml, respectively. The robustness results were found to be 98%-102%.