THE FUTURE OF PLANAR CHROMATOGRAPHY: A REVIEW ON HPTLC INSTRUMENTATION AND INNOVATION

Aakanksha Sinha* and S. J. Daharwal

High Performance Thin Layer Chromatography (HPTLC) method is a high-tech and automated version of thin-layer chromatography (TLC) with improved and superior separation efficiency and the detection limits and it is a perfect substitute for GC and HPLC. Uses of HPTLC are the phytochemical and biomedical evaluation, quantification of herbal drug, quantification of active ingredient, fingerprinting of formulations, and test for adulterants in the formulations. HPTLC effective in the detection of substances of forensic interest. Different advanced techniques in terms of HPTLC such as hyphenations in HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser have transformed HPTLC into a potent analytical tool in the analysis field. Specialists are the belief that HPTLC future to combinatorial approach and the use of instrumental HPTLC towards the analysis of drug formulations, bulk drugs, and natural products will grow in the future.