LC–MS/MS CHARACTERIZATION OF BIOACTIVE SECONDARY METABOLITES FROM TWIGS OF ARAUCARIA CUNNINGHAMII

Eneng Lulu Salamah, Kurniawanti, Auliya Ilmiawati, Purwantiningsih*

Araucaria cunninghamii is a member of the Araucaria genus known to contain diverse bioactive secondary metabolites. This study aimed to identify and characterize the secondary metabolites present in the twigs of A. cunninghamii using LC–MS/MS. Methanolic extracts of A. cunninghamii twigs were chromatographically fractionated and analyzed using LC–HRMS/MS. Fraction FAn14D revealed six compounds, including meranzin and osthol (coumarins), phenylpropanoic acid and benzoic acid (phenolic acids), 4′,4′′′,7,7′′-tetra-O-methylcupressuflavone (biflavonoid), and (2E)-3-(3,4-dihydroxyphenyl) acrylate 9,19-cyclolanost-24-en-3-yl (triterpenoid lanostanoid). Benzoic acid was the predominant compound (45% peak area. negative ionization mode). while dioctyl adipate was identified as an instrumental artifact.